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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is monitoring the emergence of resistance to colistin in Gram-negative bacteria. Resistance to colistin is often mediated by modifications of the bacterial lipopolysaccharide (LPS).
The genetic basis for acquired colistin resistance most commonly involves:

A) Alterations in the outer membrane porin proteins, reducing colistin uptake.
B) Mutations in genes encoding enzymes that modify the lipid A component of LPS.
C) Acquisition of genes encoding efflux pumps that specifically target colistin.
D) Production of enzymes that directly inactivate the colistin molecule.

2. A patient with cystic fibrosis experiences a pulmonary exacerbation. Sputum culture grows a mucoid strain of Pseudomonas aeruginosa. The mucoid phenotype is primarily due to the overproduction of:

A) Elastase
B) Alginate
C) Exotoxin A
D) Pyocyanin

3. A patient with a history of recurrent urinary tract infections caused by Escherichia coli develops a new infection. The urine isolate is resistant to trimethoprim-sulfamethoxazole. The MOST common mechanism of resistance to this antibiotic combination in E. coli involves:

A) Mutations in the genes encoding dihydrofolate reductase and dihydropteroate synthase.
B) Enzymatic inactivation of trimethoprim and sulfamethoxazole.
C) Expression of efflux pumps that specifically target trimethoprim and sulfamethoxazole.
D) Increased production of para-aminobenzoic acid (PABA).

4. A clinical microbiology laboratory is evaluating a new rapid diagnostic test for influenza viruses. The test has a high sensitivity but a lower specificity compared to RT-PCR. In a setting with a low prevalence of influenza, the use of this rapid test is MOST likely to result in a high number of:

A) False positive results
B) True positive results
C) True negative results
D) False negative results

5. A microbiology laboratory receives a corneal scraping from a patient with keratitis. Microscopic examination with calcofluor white stain reveals the presence of septate hyphae and conidiophores with a foot cell and phialides. The MOST likely genus is:

A) Mucor
B) Rhizopus
C) Candida
D) Aspergillus

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